RNA

Part:BBa_K5099017:Design

Designed by: Isabelle Guo   Group: iGEM24_McGill   (2024-09-12)


vanAB_tracrRNA_CasX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Secondary structure and tertiary structure of RNA.


Source

tracrRNA repurposed to bind to mRNA sequence of tetX antimicrobial resistance gene.

References